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KMID : 0604520040300010029
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Journal of the Society of Cosmetic Scientists of Korea
2004 Volume.30 No. 1 p.29 ~ p.32
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Inhibition of Melanin Synthesis by Enhanced Cytosolic Delivery of N-glycosylation Inhibitors Using pH-Sensitive Nano-carrier
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박주영:Park Ju-Young
최현정:Choi Hyun-Jung/심종원:Shim Jong-Won/안수미:Ahn Soo-Mi/김준오:Kim Jun-Oh/장이섭:Chang Ih-Seop
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Abstract
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Inhibition of the early N-glycosylation process in the endoplasmic reticulum prevents the activation of tyrosinase, a key enzyme for melanin biosynthesis. This work aims at evaluating the increased activity of N-glycosylation inhibitors in vitro b, employing a nano-sized pH-sensitive liposome as a delivery carrier. Melexsome, a pH-sensitive nano carrier loaded with glycosylation inhibitos, was prepared by the hydration method with phospholipids and cholresterol-based amphiphiles. Inhibitory effects of Melexsome on the N-glycosylation process were evaluated by EndoH ＆ PNGaseF digestion and the western blotting. Melanin synthesis was also monitored after treatment with Melexsome Interestingly, Melexsome effectively increased the efficacy of N-glycosylation inhibitors. Melexsome was also much more efficiently translocated into the cytoplasm as observed in CLSM. These results demonstrated that the amphiphilic lipid-based pH-sensitive nano-carriers could be, used as an efficient delivery system for N-glycosylation inhibitor to enhance the effects of skin whitening cosmetics.
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KEYWORD
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